FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859311
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC6650.020.71444145
GTATTAG16600.019.6558761
GGTAGTC7200.019.131577
TAGGTAG8350.018.233675
ACTATGC15750.017.0314148
CTATAGG7251.8189894E-1217.0019551
CTAATAC19400.016.8171353
TATACGG11650.016.80275
CCCCTAG3103.1027777E-416.372851
TAGACAG10100.015.7921955
GTTATAC14250.015.7720473
CTATGCA13850.015.7036669
TACACAG9850.015.4569675
CTACACC12200.015.4509354
GTCCCGG2900.003755569214.9997089
TAATACT29800.014.8407144
CACGAGC7353.7471182E-1014.796028145
TATACTG23700.014.6836535
GTATAAT27800.014.6059961
CCTACAC6952.7030183E-914.6044243
Produced by FastQC (version 0.11.2)