FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017413
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14500.026.001591
TATACAG22150.018.656645
CCGCTCG1950.00684441218.5890379
GTATATA39400.017.6660561
CTATACT16900.017.1595384
GAGCCCC9400.016.969543145
TAGACAG11600.016.8747795
ACTATGC15700.016.6235738
GTATATG23300.016.4924261
TAATACT30500.016.4014244
GCCCCGA3554.7044767E-516.3374088
GTATTAC17450.015.3734341
GGTAGTC8551.8189894E-1215.2625787
CTAATAC18750.015.0801753
GCTAGAC6751.7898856E-915.037213
GTCCTAG5405.010297E-714.76942351
TAGGTAG9350.014.7324275
ACTGTGC7454.6020432E-1014.5967628
TCTATAC16500.014.5001693
GTATAGG13100.014.3901941
Produced by FastQC (version 0.11.2)