FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3965494
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC28900.020.571333145
TAGGTAG13550.019.2621235
GTATTAG24100.019.2549441
CTCCGAC8600.018.546871145
ATGCCGG47450.018.335445145
TATACAG31250.017.4001185
GTATAGA26050.016.978581
TCTAGAC17150.016.9097373
TTATAGT39200.015.720774
ACTCCGC15700.015.7005468
ACTATGC30500.015.6884338
TTAGGTA22350.015.5705764
CTAATAC29050.015.4734273
CCTAATA25850.015.1453622
TATTAGG25000.014.7902882
TATATAC60950.014.7498953
CTTACAC15750.014.7302593
ATACTAT39950.014.699546
TATAAGG23500.014.5002812
TACACCG11800.014.1314525
Produced by FastQC (version 0.11.2)