FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459899
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC13250.020.246399145
TATACGG13250.020.2451635
CTAATAC22500.019.9784723
ACTATGC20050.019.1644768
CTATGCA14900.018.97649
GTATAGA17700.018.4339681
CCTAATA21650.018.419032
GTATTAG19950.018.1721651
TAGGTAG9400.017.7392585
GTATATA49250.017.2249981
ATACTAT33150.017.0587236
CACGAGC13250.016.963198145
ATACGGT13850.016.7508056
CAGCGTG7800.016.730679
TATACAG25100.016.4640465
CTTACAC11250.016.1116713
GACTAGG7651.8189894E-1216.1110158
TAATACT39300.016.0495244
ACCTAAT23950.016.0453781
TCTATAC19900.015.6663743