FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466083
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC5700.027.982325145
CCCCTGG2756.7666406E-926.365311
ACAGGGC4900.025.1529438
GTATTAG11800.022.7344951
TCCTTGC13450.021.0222078
TAGGTAG6600.019.7733085
CCGCGCA3001.1061436E-519.3332429
CCTTGCG7500.019.3332429
TATACTG20850.018.4297985
GTATATG20650.018.2578181
CTTAGAC8950.017.8217543
TCTATAC11000.017.1368683
CTTAAGG6354.1836756E-1117.1264883
GTGTATG8600.016.8615381
ACCGTGT4801.3156932E-716.6145068
GTATATA34050.016.6089841
GTATAAT23250.016.2160841
TCCTACG4109.028547E-615.9151022
CCCTATG3655.9596423E-515.891421
GGTAGTC6901.4370016E-1015.7607957