FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063154
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG11800.017.8177195
TATACGG16750.017.7461915
GTATTAG22250.016.944851
CCTAGGA6154.783942E-1016.5042612
CTAATAC26500.016.1411743
TATACAG31300.015.9823565
GAGCCCC12000.015.70929145
ATGCCGG13900.015.648434145
TACGGTG15350.015.586257
GTCTTAG13700.015.3476511
CTAGACT10400.015.3364724
CTAGGGG3406.27137E-414.9261613
CCTATAC15550.014.9193043
CCTACAC8901.8189894E-1214.6626183
CATATAC36900.014.5389953
GTATAGA24400.014.2631641
CCTAGAC10350.014.0093713
CCTAATA28000.013.982312
ACGGTGT16600.013.9758428
GTATAAG23900.013.9548241
Produced by FastQC (version 0.11.2)