FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568087
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG11900.018.2769435
TATACTG28300.017.6763235
GTGTAAG11700.017.3521041
ACTATGC17150.017.3320128
CTAATAC20700.017.1611563
TTATACT28000.017.0889424
GTATTAG17800.016.7010541
TAGGTAG10050.016.5917055
CTATACT18850.016.5381284
TATAATA79800.016.4442232
ATACTAT30950.016.3970856
TTATAGT27750.016.1978724
GTATAGG12600.016.1126691
CTATGCA14850.016.1107869
GAGCCCC14700.015.783532145
TAGTCCG16100.015.7605529
TAATACT34900.015.5799164
TAGACAG14050.015.4801165
TACTATG19250.015.4412477
GTATAGA18350.015.4102111
Produced by FastQC (version 0.11.2)