FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160427
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG3702.6011548E-1023.5163571
CTATAGG4451.1277734E-1021.1823371
TCTATAC10350.021.0134123
CACGAGC7600.020.98757145
CCTAGAC3901.2207238E-820.4476663
GTATATA29800.019.4654431
TACTCCG3401.5159912E-619.1901915
GAGTCCC1900.00588872719.0787899
CTAGGGT2307.9657463E-418.9120714
GTAGGAC2751.2429064E-418.4535983
GCGCTGC2000.00791720118.124858
ACTGTGC4659.156793E-817.1503948
TACGGTG6354.1836756E-1117.1258417
ATACGGT8900.017.1058626
CTACCCC3002.4182399E-416.9157984
GTATATG16000.016.7676541
TATACCG6959.094947E-1216.6897895
TATACAG13700.016.4042665
CTGTGCT6658.185452E-1116.3532479
CACTGTG5352.5471309E-816.2615477