FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241159
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25350.022.5952531
TAGGTAG14150.022.544345
TATACAG35800.018.4289115
ATGCCGG13550.017.65805145
GGTAGTC15100.017.2846327
TATACGG19150.017.0366825
GAGCCCC14600.016.884731145
GTATAGA28100.016.7716241
TAGTCAG15150.016.7490439
GTATATG40400.016.5110451
GTATATA65400.016.0752721
TACACCG11000.015.8183015
CTAGGGT7402.5465852E-1115.6753124
TAATACT48600.015.6632154
GTGTAGG9800.015.536791
TATTAGG28550.015.4902462
CCTGGAC8005.456968E-1215.4058923
ATACGGT20900.015.2632746
TATACTG40700.014.7851235
CTATACT28750.014.6257484
Produced by FastQC (version 0.11.2)