FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439942
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG6450.021.3571145
TATACGG9300.021.0481595
CCCACGC5301.8189894E-1220.51936145
ACAGCGC3701.5717706E-719.5937028
TACGGTG8000.018.1241767
GAGCCCC6052.0008883E-1117.975636145
GGGGTGT3002.4179983E-416.9164856
CTACGAC3956.3392145E-616.5188083
GTAGGAC3605.299763E-516.1109393
CCGTCGC4058.041507E-616.1103769
CACGAGC13600.015.99303145
GTATATA30900.015.9556281
TAGACAG8200.015.9144615
TATACAG14600.015.8902395
TAGGTAG6851.2914825E-1015.8757425
GCTATAC6408.421921E-1015.8592033
GTATAAT19400.015.3230651
ACGGTGT7603.8198777E-1115.2624638
TCTGAAC11000.015.1589283
GTATTAT24150.015.0112331
Produced by FastQC (version 0.11.2)