FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864645
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6150.025.9349785
ATGCCGG7600.022.895369145
GAGCCCC7700.022.598024145
CTCCTAC8950.021.8726981
TCCCTGC5850.021.0678278
ACACGGC2307.965429E-418.9130576
GTATAGA14750.018.187431
CTTCCTA12550.017.909341
ACTATGC14900.017.5163238
CCTCCTA5401.5133992E-917.4546531
CCCCTAC5151.592889E-816.8941231
GGTAGTC7750.016.838277
GTATTAG15200.016.6949861
GTATCCC6556.548362E-1116.6039561
CACGAGC21000.016.571886145
CTGATCG4409.681589E-716.4768439
AGACCCG4007.1456907E-616.3125115
CCTGGAC4901.6639206E-716.2759573
GTTAGAC9400.016.1972563
ATGCTAG11250.016.1119861
Produced by FastQC (version 0.11.2)