FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808016
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG19900.019.30961
GAGCCCC8000.018.126022145
GTATATA34950.017.8402841
GTATAGA14100.016.9685751
TATATAC34900.016.82623
TATACAG18650.016.7153765
CCTATAG6959.094947E-1216.6902073
TATACGG10750.016.1856175
CCTACAC5402.859997E-816.1106833
CACGAGC20100.015.871544145
ACCGCGT5152.931447E-715.4845978
CCGTGCA3757.51672E-515.465839
GTGTATG9000.015.3059971
TATACCG8551.8189894E-1215.2627545
ATGCCGG8651.8189894E-1215.087557145
GTGTAAG7755.2750693E-1114.9681741
GCCCACC6801.9845174E-914.927313145
TAGGACA7303.3833203E-1014.8968664
TATAAGG14200.014.8063552
CATATAC22650.014.7236713