FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3754678
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC15850.020.586424145
GTATTAG29400.018.7423231
GTATATA74800.017.6411511
CTATACT31000.017.0721954
TACGGTG21250.016.7172727
GCCCACC11900.016.45184145
TATACGG22450.016.1468455
ACGGTGT19800.016.1107488
ACTATGC25500.015.9212118
TAGGTAG14850.015.6227515
ATACGGT26350.015.4078276
TCTATAC27950.015.303773
TAATACT54600.014.7386794
CTATGCA23950.014.5299449
TATACAG37950.014.51897055
TCTACAC24250.014.3501933
GTATATG41500.014.32593251
GTATAAC29400.014.3033511
GTATAGA30700.014.1700071
CTAATAC34850.013.7299633