FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015909
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC27350.018.821854145
GTATAGA40200.018.0370541
TACACCG16950.017.9643145
CACGAGC30500.016.877956145
TATACCG22150.016.3654275
GTATTAG34200.016.3251171
GTATATA78450.016.0822931
TCTATAC38550.015.98547653
CTATGCA29500.015.7285619
TAGGTAG19250.015.4413115
ACTATGC34350.015.4073228
TACACAG26350.014.8574485
TATACGG28450.014.780085
CTATACT36100.014.660434
CTAATAC44800.014.56453
TATACAG46700.014.4376715
TACTATG43500.014.3331047
GTATAAT61050.013.8960661
GTATATG50250.013.8524581
CATACAC28300.013.83373
Produced by FastQC (version 0.11.2)