FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064673
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG2400.001059835918.1260051
GAGCCCC8150.017.793692145
GTATAGA19200.017.748381
TATACAG25250.016.9403025
TATATAC55350.016.895913
CCTAGAC7101.2732926E-1116.3369563
GTATTAG17550.016.1120051
CACGAGC9150.015.849026145
GTGTAGG7803.6379788E-1215.8021571
GGTAGTC10150.015.7132547
GTCCTAC4701.9245508E-615.4263881
GTATATA48900.015.4200781
TATAGGG10950.015.227312
ATACAGT22450.015.1775466
CTAATAC21100.014.77391053
GTATATG29950.014.5250121
ATATACA51950.014.5130034
ACTATGC14000.014.4990498
TAATACT35000.014.4990474
TAGGTAG9550.014.4238375