FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10221
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATC130.1271891204383133No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTT151.194894E-4145.05
GAGTATA151.194894E-4145.01
GGTTGAT100.006989024144.999982
ACAGAGC100.006989024144.999988
GACAGAG100.006989024144.999987
ATAGGTA100.006989024144.999985
CCCCGTA100.006989024144.999989
CCACTAC100.006989024144.999981
GCTAAAG100.006989024144.999988
TTTAGCC100.006989024144.999983
ACCTTTG100.006989024144.999987
TACCCTT100.006989024144.999985
GTAACAC100.006989024144.999989
CTACCCT100.006989024144.999984
GATAGCA100.006989024144.999982
TAGTACT203.756098E-4108.749994
CCTTTGT301.5244208E-596.6666648
GTATTAT259.120734E-487.01
TATTATT259.120734E-487.02
CTTTGTA259.120734E-487.09
Produced by FastQC (version 0.11.2)