FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826020
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA68550.018.1924381
ACGGTGT17100.016.9585118
GTATTAG24100.016.8477131
TATACGG20750.016.7717255
TAGGTAG11800.016.5896175
GTATATG39000.016.3601781
TATATAC77300.016.1325683
ACTATGC21900.015.5588538
TACGGTG17800.015.4770247
TAATACT45600.015.4227384
TATACAG36300.015.3793755
GTATAAG23750.015.2642811
TCTATAC25350.015.158373
GAGCCCC10050.015.1501145
CTAATAC22750.014.9785943
GTATAGA25750.014.6418531
TACACAG18450.014.539855
CATATAC41500.014.3258493
ATACGGT21900.014.2349476
CACGAGC13300.014.173724145