FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_vs32_171218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_vs32_171218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10023953
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG22100.016.1656631
GATCGGA34100.011.028281
GAGCACA45200.08.6298559
TGGGGGG30150.08.2634361
AGAGCAC49750.07.840678
GCACACG26200.07.174974410-11
ACACGTC28900.07.07366412-13
TCGGAAG57550.06.7790723
CACACGT31150.06.487291312-13
AGTTCCG32250.06.411836634-35
CCGTATC29050.06.390045638-39
ACGTCTG30950.06.37756114-15
CGTCTGA33100.06.247431316-17
TGCCGTC30100.06.245373250-51
GCCGTCT26200.06.18846250-51
CACGTCT33000.06.123793614-15
GAACTCC38050.05.86706920-21
GTTCCGT36050.05.735970534-35
CGTATGC32000.05.72760546-47
GTCACAG39050.05.71646828-29
Produced by FastQC (version 0.11.2)