FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_vs31_171218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_vs31_171218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9561515
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36400.016.7892651
GAGCACA42950.014.6619849
AGAGCAC48750.013.4961248
TCGGAAG52950.011.9837193
GCACACG28400.010.92138910-11
TGCCGTC31450.010.16122850-51
ACGTCTG31400.010.02776614-15
ATCGGAA66000.09.9706052
CACACGT32600.09.802612-13
ACACGTC31650.09.65138412-13
GCCGTCT31500.09.54832950-51
AGGGGGG26100.09.45332774-75
CGTCTGA34100.09.37169516-17
CACGTCT34950.09.21090914-15
CGGAAGA72450.09.1475934
CGTATGC35550.08.98923446-47
GTATGCC36950.08.58504846-47
CACAGTC37850.08.50514130-31
TATGCCG38250.08.41606548-49
CAGTCAC38850.08.40707526-27
Produced by FastQC (version 0.11.2)