FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_vs30_171218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_vs30_171218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11345389
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG31000.09.2499195
GATCGGA35450.07.69192651
TTGGGGG31300.07.50922974
GAGCACA46250.06.4014169
AGAGCAC48900.05.95843178
AGGGGGG23050.05.91494131
GCACACG24550.05.838384610-11
GCCGTCT25650.05.404947348-49
TCGGAAG53000.05.14422463
ACACGTC27250.05.087435712-13
Produced by FastQC (version 0.11.2)