FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_vs29_171218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_vs29_171218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10961681
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA51400.022.408441
GAGCACA55950.022.0066179
AGAGCAC62950.019.4103628
TCGGAAG71350.017.129413
GCACACG38500.015.68546710-11
ACACGTC41450.014.90929912-13
ATCGGAA85600.014.4977682
CACACGT43700.014.1416612-13
CGGAAGA89050.014.0942634
CACGTCT46100.013.8135514-15
ACGTCTG45600.013.34663814-15
CGTCTGA46900.013.17725116-17
CAGTCAC48900.012.5416826-27
TCCAGTC50950.011.9909424-25
CATCTCG34650.011.93541338-39
CCAGTCA53700.011.81445126-27
AGCACAC52700.011.77113610-11
AGTCACC55600.011.53753328-29
CCAGATC56600.011.2507632-33
TGCCGTC36550.011.12202348-49
Produced by FastQC (version 0.11.2)