FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_vs28_171218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_vs28_171218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11026375
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG30650.08.1283335
AGGGGGG27950.07.40062331
TTGGGGG30300.06.36064624
GATCGGA27303.6379788E-125.8548221
GTCACGC14601.2732926E-105.31112728-29
AGAGCAC41850.05.05327228
Produced by FastQC (version 0.11.2)