FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_vs27_171218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_vs27_171218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9597465
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA38150.032.4100841
GAGCACA48800.027.445989
AGAGCAC54200.024.711648
TCGGAAG58650.023.2422813
GCACACG33050.020.26242610-11
ATCGGAA70250.019.4721322
ACACGTC36700.018.31115512-13
CACACGT38550.017.55431612-13
CGGAAGA76850.017.4933594
CACGTCT39250.017.36097514-15
GTCACAC39400.016.99687228-29
ACGTCTG40450.016.84594214-15
CACACAG40300.016.5592430-31
CGTCTGA41500.016.13670316-17
CAGTCAC44600.015.331161526-27
TGCCGTC37850.015.02353448-49
GCCGTCT35250.014.86511748-49
TCCAGTC46250.014.784018524-25
GAAGAGC94400.014.7391686
CGTATGC41350.014.3769444-45
Produced by FastQC (version 0.11.2)