FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_vs26_171218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_vs26_171218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9595177
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG27600.09.3683371
GATCGGA19800.07.1230321
TGGGGGG25950.06.8833295
GAGCACA31850.05.90205539
TTGGGGG25851.4915713E-105.63709974
Produced by FastQC (version 0.11.2)