FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_vs25_171218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_vs25_171218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172998
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36350.019.4001851
GAGCACA42200.017.4840839
AGAGCAC44450.016.599068
TCGGAAG53200.014.049383
GCACACG27700.013.06367610-11
ACACGTC29550.012.64316512-13
ACGTCTG30300.012.25263414-15
ATCGGAA63600.011.60436252
CACACGT32450.011.36844712-13
CGTCTGA33550.011.06574616-17
CACGTCT34350.010.94481114-15
TGCCGTC27550.010.9172148-49
CGGAAGA70400.010.6169194
CCGATGT34150.010.59650732-33
CAGTCAC37500.010.27633526-27
GCCGTCT26800.010.17059948-49
GTCACCG37500.010.15104128-29
GTATGCC33150.09.99450344-45
CGATGTA38550.09.87468734-35
CGTATGC34700.09.75121444-45
Produced by FastQC (version 0.11.2)