FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah8220spike-inp_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah8220spike-inp_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10006559
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT674080.673638160730377TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT101550.034.4539350-51
TGCCGTC107250.032.9733650-51
TATCTCG108450.032.1750940-41
GTATGCC110600.032.03827346-47
CGTATGC113950.031.07576646-47
TATGCCG115200.030.8810448-49
ATGCCGT116100.030.66189248-49
CCGTCTT118600.029.64053752-53
CTCGTAT120800.029.1385444-45
ATCTCGT121100.029.04709442-43
TCGTATG124600.028.62704844-45
TCTCGTA123750.028.4060942-43
AGGGGGG86400.027.25180874-75
CGAGTGG130900.026.90861932-33
CTGCTTG130550.026.80123558-59
CACGAGT134350.026.40994830-31
ACGAGTG135250.026.25164832-33
GAGTGGA144600.024.76531834-35
AAAGGGG95550.024.49445772-73
TCACGAG147850.024.0461730-31
Produced by FastQC (version 0.11.2)