FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah8220spike-chip_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah8220spike-chip_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12136998
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT802770.6614238545643659TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT115150.035.40493450-51
TGCCGTC121350.034.44803250-51
ATGCCGT129250.032.74199748-49
TATGCCG129950.032.71028548-49
GTATGCC131400.032.42085646-47
CGTATGC133000.032.10149846-47
AGGGGGG95350.030.78124274-75
CCGTACG142750.030.60027132-33
CGTACGT143850.030.36640434-35
CTCGTAT135600.030.23832744-45
CCGTCTT136950.030.02746252-53
TCGTATG144650.028.98000544-45
ATCTCGT143700.028.22318842-43
GTACGTA155300.028.21832534-35
TCTCGTA146200.027.94951442-43
AAAGGGG105450.027.16370472-73
CTGCTTG145750.027.0370958-59
CGTAATC154850.026.90405738-39
ACCGTAC166950.026.23502732-33
AATCTCG156750.026.12823540-41
Produced by FastQC (version 0.11.2)