FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah8219spike-inp_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah8219spike-inp_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9314161
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT99740.10708425589808894TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG71750.021.51689132-33
GTTTCGG75500.020.57283434-35
AAGGGGG51050.020.25338774-75
AGTCACG81800.019.01628328-29
GTCACGT83400.018.76415828-29
CGGAATC84950.017.84106338-39
AATCGCG28650.017.46964540-41
CAGTCAC93400.016.78012726-27
GAGCACA188650.016.6910219
TCGGAAT94250.016.67919236-37
AGGGGGG49450.016.48936574-75
AGAGCAC200450.015.7555228
TCACGTT102550.015.65011930-31
ACGTTTC102100.015.51208732-33
CCAGTCA101300.015.49470426-27
TCCAGTC101400.015.47934124-25
TTTCGGA102100.015.42015234-35
CACGTTT102350.015.38223930-31
TTCGGAA104000.015.04773536-37
GTATGCC41050.014.08141846-47
Produced by FastQC (version 0.11.2)