FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah8219spike-chip_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah8219spike-chip_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10297465
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1337981.29932949517187TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA332900.042.2934579
AGAGCAC350200.040.2582178
TGCCGTC170200.039.22269450-51
GCCGTCT164800.039.13910350-51
GTATGCC175300.038.48296746-47
TATCTCG173850.037.6549140-41
ATGCCGT178350.037.56100548-49
TATGCCG181950.037.25691248-49
GGCCTTA186150.037.18691636-37
CGTGGCC187500.036.9322432-33
CGTATGC183650.036.81003646-47
CTCGTAT178900.036.72368244-45
CCGTCTT179750.036.3290352-53
ACGTGGC192750.036.31640632-33
AGGGGGG132000.036.1544574-75
CACGTGG193600.035.93840430-31
TCGGAAG398700.035.355383
CGGAAGA399400.035.270224
TCGTATG187750.035.1052744-45
TCACGTG200200.035.07052630-31
Produced by FastQC (version 0.11.2)