FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah8218spike-inp_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah8218spike-inp_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300817
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC96990.11684392030326654TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG29250.018.79814528-29
CACGGCT29400.018.3826530-31
TCACGGC30950.018.1453330-31
GAGCACA62650.017.4775549
CGGCTAC31550.017.20437632-33
GCCGTCT32050.016.93587548-49
CATCTCG32800.016.40519538-39
TGCCGTC33850.016.38238148-49
AGTCACG34100.016.19341728-29
GTATGCC35700.015.59925444-45
ACGGCTA35800.015.55577432-33
AGAGCAC71700.015.4683488
CGTATGC38400.014.86958244-45
AGGGGGG27950.014.54444472-73
GATCGGA75550.013.7522491
TATGCCG41900.013.40325246-47
GGCTACA41300.013.31349634-35
TCGGAAG86550.013.0345873
ATGCCGT44100.012.73460946-47
CTCGTAT43900.012.47132242-43
Produced by FastQC (version 0.11.2)