FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah8218spike-chip_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah8218spike-chip_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10989230
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC199710.18173247807171203TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG46400.024.61281872-73
GAGCACA156250.024.3922679
CACTAGC79350.023.92718930-31
GTATGCC56950.022.73458944-45
TGCCGTC48050.022.39733748-49
AGAGCAC173300.022.1011228
CAGTCAC87800.021.65067126-27
TATGCCG60100.021.3474746-47
CGTATGC58050.021.33229644-45
ACTAGCT90850.021.10568232-33
GCCGTCT43550.021.04266548-49
TATCTCG59250.020.93990938-39
TCACTAG92100.020.89544130-31
TCCAGTC92650.020.54286224-25
CCAGTCA93200.020.39623326-27
GCTTATC90400.020.37890436-37
TAGCTTA94500.020.34017634-35
AAGGGGG60950.020.27939872-73
AGTCACT95200.020.09119628-29
CTAGCTT95250.020.05671132-33
Produced by FastQC (version 0.11.2)