FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah7011spike-inp_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah7011spike-inp_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9379276
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC734460.7830668379947451TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA200250.039.731959
AGAGCAC209400.038.108038
TGCCGTC109450.035.9179448-49
GATCTCG104100.035.7996738-39
GCCGTCT109950.035.64774348-49
CGATCAG112300.035.42516732-33
GTATGCC113300.034.8012244-45
GTCACGA116450.034.32321528-29
CACGATC117150.033.9381530-31
AGTCACG117350.033.87976528-29
CGTATGC118300.033.46936844-45
TATGCCG119350.033.0175946-47
CTCGTAT116150.033.01667842-43
ATGCCGT120150.032.8955346-47
AGGGGGG88700.032.85041872-73
TCGGAAG245750.032.420323
ATCTCGT118300.031.6415440-41
TCGTATG125350.031.53073142-43
TCTCGTA122300.031.24069640-41
CCGTCTT125750.031.1694950-51
Produced by FastQC (version 0.11.2)