FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah7011spike-chip_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah7011spike-chip_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11225838
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC271040.2414429996228344TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA194150.035.025469
AGAGCAC207750.032.778268
GTCACAC107900.031.29336428-29
CAGTCAC120900.028.08396526-27
AAGGGGG81150.027.50914672-73
AGTCACA125000.027.3507928-29
CGGAAGA250150.027.284234
TCGGAAG251200.027.0952133
CCAGTCA126250.026.91248726-27
TCCAGTC126550.026.8301824-25
GTATGCC69900.026.42261544-45
AGGGGGG71900.026.2439272-73
TCACACT132600.026.04932830-31
GATCGGA261200.024.9640251
GAACTCC136000.024.9488220-21
TGCCGTC56400.024.87235848-49
GAAGAGC277900.024.5770846
ACACTTG139950.024.56421332-33
ACTCCAG138150.024.45811322-23
TATGCCG72750.024.45059646-47
Produced by FastQC (version 0.11.2)