FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah119spike-inp_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah119spike-inp_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9591536
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC196250.2046074789272542TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC49050.025.72485232-33
ACTTAGG55150.022.92209632-33
GCCGTCT46300.022.02581648-49
CATCTCG49650.021.67506638-39
GTATGCC52950.021.47832344-45
TGCCGTC50650.021.10839548-49
GAGCACA125500.020.557519
TTAGGCA61850.020.55305134-35
TAGGCAT60950.020.08550334-35
TATGCCG56150.019.71040346-47
AGGGGGG46050.019.54335872-73
CGTATGC58550.019.38390244-45
ATGCCGT58750.019.15808346-47
AGGCATC63000.018.87256636-37
AGAGCAC137150.018.7770218
AAGGGGG46900.018.23721972-73
CACTTAG72250.018.21237830-31
GCATCTC60100.017.98446738-39
CAGTCAC73950.017.50791526-27
CTCGTAT61400.017.45079442-43
Produced by FastQC (version 0.11.2)