FastQCFastQC Report
Thu 21 Dec 2017
HW373AFXX_n01_ah119spike-chip_122017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW373AFXX_n01_ah119spike-chip_122017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11180082
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1597461.428844618492065TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA371250.048.0899779
AGAGCAC393550.045.3893138
TGCCGTC214950.041.2777848-49
CGATCTC201750.040.69370738-39
GCCGTCT216900.040.66834648-49
GATCTCG205900.039.7251538-39
ATGCCGT223800.039.61398346-47
CGGAAGA451650.039.6014674
TCGGAAG453550.039.414843
GTATGCC224800.039.3750544-45
CACGATC209000.039.29341536-37
CGTATGC226350.039.2299944-45
TATGCCG227750.039.04042446-47
AGGGGGG170750.039.02771872-73
GAAGAGC467650.038.3373646
TCGTATG234000.037.7565942-43
CCGTCTT234400.037.7324350-51
CATCACG235300.037.7273232-33
CTCGTAT228450.037.58358442-43
AAGAGCA478750.037.497857
Produced by FastQC (version 0.11.2)