FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n02_zt2ss90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393547
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31090.2230997591039269No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT24440.1753798041974903No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA20540.14739366522980565No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC19590.14057652881460045No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG18280.13117605649468586No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC18070.12966911055027208No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC15650.11230335252417033No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC14100.10118065626778287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4650.038.735263
CGGTAAC1600.034.49139425
GCGAGAC6750.034.3630836
GTTCCGA7050.032.225541
TCCCATC6100.031.65740622
CCGTTCT5400.031.4219444
TTCCCAT6050.031.34905421
CTACTAA5750.030.393321
TTCCGTT5950.030.2742792
CTTCCCA6200.030.03333720
ACTCGTC4200.029.55767823
GTCTCGC3550.029.273882
AGACCAC6250.028.69065322
CTTCCGT5150.028.5048031
GACCACA5950.028.39855623
CCCATCT6900.027.98698223
CTCCTAG2150.027.6367151
GTTCCGT2300.027.3539891
GGAAGAC5850.026.52612319
GAAGACC6500.026.5251720
Produced by FastQC (version 0.11.2)