FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n02_zt2ss63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369904
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37560.2741797965404875No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC5900.056.3192143
GCGAGAC13950.042.664266
TCCCATC11450.040.3640222
TTCCGTT8150.040.3492282
GTTCCGT4200.039.041281
CGTTCTC2600.038.598155
GTTCCGA11950.038.5373231
GTCTCGC8150.038.2255862
CCCATCT12900.035.55961223
CTTCCCA13100.035.01799820
CACTCGT6300.034.4901522
CCGTTCT9400.034.2421154
TTCCCAT13750.033.86305621
GCACTCG6700.032.43103821
GACGTCT12350.032.39679320
GACCACA7500.032.19080723
AGACGTC12250.032.09813319
TCGTCTC6450.032.08620525
AGTCGTG3700.031.7994045
CGTTCTG6000.031.7214665
Produced by FastQC (version 0.11.2)