FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n02_zt2ss57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572553
Sequences flagged as poor quality0
Sequence length75
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20339712.93419045335833No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG374502.3814777625936934No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290571.847759662154471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166831.060886342145543No Hit
CGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163341.0386931314874603No Hit
GGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85740.5452280463679126No Hit
CGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51960.3304181162733466No Hit
GGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28520.18136113695373066No Hit
GGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20370.12953458484388125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT16150.039.4914972
GGCCGGG28050.038.2832955
CTTCCGA16050.036.8173031
GCCGGGG30600.035.766526
CTTCCGT2350.035.7094961
TCCGTTC2900.034.6590463
TCCGATC19350.033.8530243
CGGGGCG26800.033.3192448
CCGATCT18100.032.7399944
CGGCCGG16950.032.712324
GGGGCCG17600.032.4928553
GGGGGCC17950.030.7037182
GTTCCGA3200.030.5948541
TCCGTAC802.6651996E-630.3266663
CGGGGGC81200.030.0135291
TCTTCCG6700.029.9870475
CGATCTG11250.029.8675565
GGGCCGG18800.029.4932884
CGGCGGC46850.029.1814921
CCGGGGG23400.028.7142147
Produced by FastQC (version 0.11.2)