FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n02_zt2ss22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894493
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12290.13739626805352306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4000.051.0669823
CCGTTCT4750.043.727744
TTCCGTT4950.041.9703942
CTTCCGT3650.041.126911
TCCCATC5500.035.74207322
GTTCCGT2650.035.5687331
CTTCCCA5850.034.19319520
ACTCGTC4300.033.6859223
GCGAGAC7100.033.6350866
TCGTCTC3750.033.11771825
GTTCCGA5600.033.039871
TTCCCAT6200.031.70667821
CGTTCTG2950.031.6805235
GCACTCG4550.031.07706821
AGACGTC6450.030.47773719
TCTTCCC6150.030.28211819
TCGGTAG3100.030.1543713
GACGTCT6600.029.7850620
CTCGTCT4650.029.67537324
GTCTCGC3450.029.1055562
Produced by FastQC (version 0.11.2)