FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n02_zt2ss1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918613
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG153991.6763315999229274No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26030.28336198159616727No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC3150.035.176913
CTTCCGT2700.033.6299321
GCGAGAC5300.031.3416886
CCGTTCT3500.030.661484
TCATCCG5500.030.2052925
TTCCGTT3750.029.5518382
GTCTCGC3300.028.3345052
GTTCCGA3650.026.790551
GGCCGGG3600.025.9576725
ACTCGTC2950.025.71820823
CTTCCCA4450.025.57372520
CGGGGCG3150.025.177358
TGAACCG2100.024.7215925
ACTGTCC1701.6916601E-1024.3402528
CATCCGA6550.024.3037626
GGGGGGC23000.023.8401719
TCTTCCC3800.023.59553319
TCCTACA2500.023.549122
GCCGGGG4000.023.3593526
TTCTAAG3000.023.0873722
Produced by FastQC (version 0.11.2)