FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n02_zt2s60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTGGGAGAGCGTAATTTACGCGCCTGCTTCCTTCCTTAGATGTGGTAGCCGTTTCTCAAGCTACCTCTCCGA18.333333333333332No Hit
GACGAGACCCGTCGTTTGTACTTGCGCCGAAACCCCCGAGGCTGGATACAGCAGGAGACCGACTATTAAACCCCC18.333333333333332No Hit
TGAATTATCCACAACGTGCTGCCGTGCCCGATGCAGTTGCCAAGTGCCGATCGGCCGGCATCAAGGTCATCATGG18.333333333333332No Hit
CTCTAGAACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACATTTGATGGTTGTATCTAGGACTACCATGGT18.333333333333332No Hit
CTAAGACTAATTAGCAAAAAAAAAACACGACAATCGTCGACAAGACTGTGAATGCCACGAACGATAGCGACACTA18.333333333333332No Hit
CTCAATTACAGTCTATTAAATTAAATTAAATTCAGTTACAAACCAATTGATTACATTACACACGGTTACACTCAA18.333333333333332No Hit
CGCAGGATAATTCCTTGAACCCAGCAGGCAGAGGTTGCCTTGAGCAGAGATGGCACCAAAGCACTACAGCCTGGG18.333333333333332No Hit
TAAATAAGTAATGTATTTGAGATTTTTTATGCGACTGAAGATTATGAAATAATTTAGTAAGGGCTGCTGTCATGG18.333333333333332No Hit
ATGCTCGCGAAAGAAACGCTCGTAAGCAGCAATTTGCCTGAATAAATAATAATCGCAAGTGACAACGAGGATTAC18.333333333333332No Hit
ATATGGAAATTCATATTGGCACTCAGGTGGTGGCGGGAAATGTGTGTAACCTATTGGCCTCCCTACATGCGGCAT18.333333333333332No Hit
CCTTGGTCTCCTGCTGCAACTGTAGATGGAAGTCGCACGGCAGATCCAGCTCCGACAAGCACGGAATCGACCGGT18.333333333333332No Hit
GTTCTGCTTTCATTGGTTATCAGATCAAGAGGTATTGATTAATAGAATCAGTTTGGGGGCAGTAGTATTACGACG18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)