FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2ss89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396919
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG438301.2902868746649536Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG195030.5741379173303809Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG146490.4312437240923319Illumina PCR Primer Index 7 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG143290.42182342293119146RNA PCR Primer, Index 41 (96% over 31bp)
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG91840.2703626433247305Illumina PCR Primer Index 7 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG61530.1811347282640534Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG46980.13830179642199297TruSeq Adapter, Index 7 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG40250.11848972554246952RNA PCR Primer, Index 41 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG268200.027.95686369
GCCGTCT208700.024.9112346
AAGGGGG230500.024.81995869
GTCGTAT28950.024.78745339
TCGTATG207400.024.78532840
TATGCCG209700.024.61146743
CGTATGC212150.024.31169941
CCGTCTT217250.024.2484347
GTATGCC216200.024.17471142
TGTCGTA30000.024.14989338
ATGCCGT215250.023.92880444
CGTCTTC215950.023.83526448
TGCCGTC222100.023.70338445
GTCTTCT210400.023.6933549
AAAGGGG242850.023.50092168
AAAAGGG247750.023.34252567
GCTTGAA200200.023.31548756
TCTTCTG216750.023.06288550
CTTCTGC212450.023.05876251
TGCTTGA197550.022.87731255
Produced by FastQC (version 0.11.2)