FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2ss85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590626
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17910.11259717872083067No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG16540.10398421753448014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATG2850.025.42581469
GTATGCC5000.024.83918442
GGTCTGA4650.024.48845769
TATGCCG5100.022.99924543
CGGTCCA7350.021.5911310
GCCGTCT6350.021.18828246
TCTAGGG5150.020.7682692
GTAGACT3500.020.69932246
GACGGTC7850.020.6553738
GTCTTAC8700.020.62521
TGCCGTC6050.020.52825245
GGTCCAA7450.020.37517211
AGTCTGA5450.020.26067469
CCGTCTT6350.019.55841447
GACGGGT11600.019.33551668
ATGCCGT5900.019.29597744
ACGGGTG11350.019.15336669
GCGTCGT7950.019.09371432
AGACGGG17650.018.96389267
GATCTTT41450.018.8932296
Produced by FastQC (version 0.11.2)