FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2ss82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108027
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG2870.26567432216020065RNA PCR Primer, Index 29 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.16477362140946245No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG1410.13052292482434946RNA PCR Primer, Index 29 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACT200.00732920851.771194
CATACTG200.00732920851.771195
TCGTGTA200.00734264851.74722358
ATATATC200.00734264851.74722337
ACTCCAA200.00734264851.74722327
AGAATAG200.00734264851.74722366
CGTTATA350.001342761639.42645661
TGGGGTG601.32761415E-534.514135
AATTTCG501.8508872E-434.4981542
CCTATCT400.002586656734.4981533
CCCGACA400.002586656734.4981532
AGACTGG652.3030827E-531.84444664
TGGACTC450.004595139530.6792245
CCTGTAC450.004595139530.6792243
AGACTCG450.0046055730.6650259
TTAGCCG450.0046055730.6650229
ACGGGGC450.0046055730.665028
TACCTTA450.0046055730.6650250
TCCGAAA703.8205613E-529.56984116
AGACGGG1103.6008714E-828.22575867
Produced by FastQC (version 0.11.2)