FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2ss78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337140
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT22790.1704383983726461No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA22260.1664747146895613No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC20130.15054519347263562No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC16920.12653873192036735No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG16520.12354727253690713No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC16470.1231733401139746No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG14650.1095621999192306No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA14360.10739339186622195No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA14350.10731860538163542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4100.026.92588244
GTATGCC4000.026.73655942
GCCGTCT5100.024.35208346
TGCCGTC5200.023.88377645
GGTCCAA8200.023.56014611
CGTATGC4550.022.74645241
GACGGTC8700.022.6026528
TATGCCG4950.022.30224443
TATCTCG4800.022.28046436
CCGTCTT5800.022.00784747
CGGTCCA8800.021.95377210
TCGCGTC8600.021.66202730
ACGGTCC9050.021.3473159
TTGTCTC10900.021.20567510
GTCTTAC10300.021.1075131
TTACGAC14100.020.797144
TCTAGGG2501.8189894E-1220.7023662
GTCTTCT5500.020.69927249
GCTTGTC11850.020.6701568
ATCTAGG10550.020.6073341
Produced by FastQC (version 0.11.2)