FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2ss63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369904
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAATAGAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGACAGTCGG15810.11540954694635537No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCTGATTAATGAAAACATC14120.10307291605835155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC9800.029.9229842
ATGCCGT10250.027.59954844
ACGGTTG500.00773604727.59954640
TGCCGTC10300.027.4655745
GCTTGAA10200.025.7054656
TGCTTGA10050.025.05929255
TCGTATG11800.024.26655240
CTCGTAT8850.024.16909639
CTTCTGC12150.023.56751451
GCCGTCT11750.023.19536646
CTGCTTG10600.022.78264654
TATGCCG12650.022.63599243
TGCGCTA4650.022.257710
AGCGATC11050.022.16705933
TATGCAA5250.021.685365
GAGACGG12250.021.40763966
TCTTCTG13300.021.27032950
ACGGGTG6350.021.19249769
TATTATG5950.020.8766542
GTATTAT6200.020.5936341
Produced by FastQC (version 0.11.2)