FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2ss62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089933
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20330.18652522677999472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC6000.031.0490742
TATGCCG5900.029.82114243
CGTATGC6000.029.32412341
CCGTCTT6000.028.7491447
TCGTATG6400.027.49136540
GCCGTCT6200.026.70887846
TGCCGTC6500.026.53766845
TGCTTGA5850.025.9479455
GTCTTCT6100.025.45005849
ATGCCGT6800.024.8595544
CTTGAAA6850.024.1744657
GCTTGAA6950.023.33023656
GGTCGCT2400.023.00247869
CTTCTGC8750.022.86788751
AGACGGG12550.022.26933167
TGTCGTA1906.7484507E-1021.7888238
TAGTGTC1808.581992E-921.0827039
CTGCTTG7250.020.93730454
GATCTTT32550.020.6685336
ATCTTTT31650.020.2752047
Produced by FastQC (version 0.11.2)