FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2ss52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4438533
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT96260.21687345796460228No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA94380.21263782425409478No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG80970.18242513911691094TruSeq Adapter, Index 7 (96% over 28bp)
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC78390.17661240774823572No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC64620.1455886438154228No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC61980.1396407326474761No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG60560.1364414773980502No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA58020.13071886589555604No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG57790.13020067666501522No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA49050.11050948590446438No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC48280.10877467848047992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC35400.025.728142
CCGTCTT35000.025.43072147
GCCGTCT36650.024.75647446
ATGCCGT36800.024.37432344
TGCCGTC37100.024.36320545
CGTATGC36450.024.32470141
TATGCCG36900.024.30826843
ACCGGTA27650.023.83116515
CTCGTAT36050.023.0634239
GCTTGAA36950.023.06160556
TGCTTGA35650.023.03161855
CCGGTAA29100.022.99936116
GTCTTCT39100.022.3229149
ATATAAG32350.021.9726872
AAAGGGG48800.021.35342668
TCTCGTA39750.021.3505938
ACGGGTG16550.021.26584469
AAGGGGG48250.021.0247369
GGTAATA32400.020.9762718
CGGTCCA25600.020.88809210
Produced by FastQC (version 0.11.2)