FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2ss21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774288
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT14640.18907693261422107No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA13110.1693168433451119No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG11580.14955675407600272TruSeq Adapter, Index 12 (96% over 29bp)
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC11140.14387411402475564No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG9950.1285051557043374No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC9610.12411402475564648No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA9310.12023949744797802No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA9020.11649412105056516No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG8650.11171553737110738No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC8240.10642035005062715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA451.0013125E-438.33300860
ACCGGTA5500.025.71796415
ATATAAG5550.025.491212
GTATATG1151.6908652E-624.00911
GTACAAT902.1200316E-423.0087221
ATCCTAC1352.6472298E-723.008721
CATGGTT3900.022.99980713
ATATCGC1352.6571433E-722.99980539
CCGGTAA6200.022.25787716
CTACGCT953.0645452E-421.789299
GACGCAT800.002898728221.56231960
GAATCAG4200.021.35696235
GTATGCC4850.021.34002742
TATAAGA6650.021.2704983
CATATAA6600.020.9170191
GGTCCAA4300.020.8602911
TAATACT6450.020.8602894
TGCAACG4150.020.78295719
CACCGGT6500.020.69982514
ATGCTTG5550.020.513346
Produced by FastQC (version 0.11.2)