FastQCFastQC Report
Sat 23 Dec 2017
HW32WAFXX_n01_zt2s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGGTGAGTATCACGTGAGTAACCTGCCCTTGACTCTAGCATAACCCAGGGAAACTGCGTCTAATACTGGAT17.6923076923076925No Hit
ATGCGATGGCTTGCAGTCTCTGGCTTTCTGCCTGCATTTTATTTGGCTTCTCGTGGCCTGTCTCTTATACTCATC17.6923076923076925No Hit
TAGGGAAACCGAATCCTAATGGGGTGTAAACTGGCATGTCCTCGACCCGAAGCGGCGTGATCTACCCATGGCCAT17.6923076923076925No Hit
GCTCTTCCGATCTGCCGAGGGAGACCTGTGCTAATCCGATCAGCCGAGGGAGACTTGAGCTCTAACAATCATATG17.6923076923076925No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT17.6923076923076925No Hit
ATAAGAAACTCCACCGGTAATACGCTTACATACATAAAGCTATAGTACTAACCACAATTGTAAGTTGTACTACCC17.6923076923076925No Hit
CGCTTACATACATAAAGGTATAGTAATAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTACAACTA17.6923076923076925No Hit
GGCGTCTCCAGCGTTGATCGGAAGAGGAGAGGAGTCGTGGGTACATGTGAAGTCCTCACGTCACGGCCGAAGGTC17.6923076923076925No Hit
GTCTGCCCGTAGCAGATCGGAAGAGCACACGTCTTCACGTATCAGAACTGATCCGCACACGACAGCAAGTAGCAA17.6923076923076925Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCCACCGGGAGACGTGTGCTCTACCGATCTCCACCGGGAGACGTGTGCTCTTCCGATCTCCACCGGGAGACGT17.6923076923076925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGTAGTTAGCCGTGACTTTCTGGTTAGATACCGTCAAGGCGCGAGTAGTTACCTAGCACGTGTTCTTCTCTAA17.6923076923076925No Hit
AAGACGCACTGCGCAAGCGAAAAGCTGAGGAAGAAGCGGTGGATGCCAGAGGAGTTTAGTATAAGAAGAATGCAC17.6923076923076925No Hit
TTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTTATCATTACCT17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)